LOCUS       sequence-164343-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac cassette contains Neomycin, puromycin and TK genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Ye L, Wang J, Beyer AI, Teque F, Cradick TJ, Qi Z, Chang JC, Bao G, 
            Muench MO, Yu J, Levy JA, Kan YW
  TITLE     Seamless modification of wild-type induced pluripotent stem cells to
            the natural CCR5Delta32 mutation confers resistance to HIV 
            infection.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9591-6. doi: 
            10.1073/pnas.1407473111. Epub 2014 Jun 9.
  PUBMED    24927590
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1407473111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-07-1- 1"; volume: "111"; issue: "26"; pages: "9591-6"
COMMENT     Sequence Label: pCAG-Neo-PuroDTK
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   6..40
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        414..793
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 attaacccta gaaagatagt ctgcgtaaaa ttgacgcatg cattcttgaa atattgctct
       61 ctctttctaa atagcgcgaa tccgtcgctg tgcatttagg acatctcagt cgccgcttgg
      121 agctcccgtg aggcgtgctt gtcaatgcgg taagtgtcac tgattttgaa ctataacgac
      181 cgcgtgagtc aaaatgacgc atgattatct tttacgtgac ttttaagatt taactcatac
      241 gataattata ttgttatttc atgttctact tacgtgataa cttattatat atatattttc
      301 ttgttataga tatcaactag aatgctagca tgggcccatc tcgacctact tcgtatagca
      361 tacattatac gaagttatgt gcgggcctct tcgctattac gccagcgcgc gttgacattg
      421 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      481 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      541 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      601 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      661 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      721 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      781 cgctattacc atgg
//
