LOCUS       sequence-164344-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac cassette contains Neomycin, puromycin and TK genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Ye L, Wang J, Beyer AI, Teque F, Cradick TJ, Qi Z, Chang JC, Bao G, 
            Muench MO, Yu J, Levy JA, Kan YW
  TITLE     Seamless modification of wild-type induced pluripotent stem cells to
            the natural CCR5Delta32 mutation confers resistance to HIV 
            infection.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9591-6. doi: 
            10.1073/pnas.1407473111. Epub 2014 Jun 9.
  PUBMED    24927590
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1407473111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-07-1- 1"; volume: "111"; issue: "26"; pages: "9591-6"
COMMENT     Sequence Label: pCAG-Neo-PuroDTK
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(146..745)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(226..246)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ccagcacctg ccagtaagtc atcggctcgg gtacgtagac gatatcgtcg cgcgaaccca
       61 gggccaccag cagttgcgtg gtggtggttt tccccatccc gtgaggaccg tctatataaa
      121 cccgcagtag cgtgggcatg gatccggcac cgggcttgcg ggtcatgcac caggtgcgcg
      181 gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga
      241 ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca
      301 ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga
      361 cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct
      421 gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga
      481 acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt
      541 ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct
      601 cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg
      661 cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca
      721 ccgtgggctt gtactcggtc cccatggttg tggccatatt atcatcgtgt ttttcaaagg
      781 aaaaccacgt ccccgtggtt cggggggcct agacgttttt ttaacctcga ctaaacacat
      841 gtaaagcatg tgcaccgagg ccccagatca gatcccatac aatgggg
//
