LOCUS       sequence-164354-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human RagA from a CMV promoter for stable expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Efeyan A, Schweitzer LD, Bilate AM, Chang S, Kirak O, Lamming DW, 
            Sabatini DM
  TITLE     RagA, but not RagB, is essential for embryonic development and adult
            mice.
  JOURNAL   Dev Cell. 2014 May 12;29(3):321-9. doi: 
            10.1016/j.devcel.2014.03.017. Epub 2014 Apr 24.
  PUBMED    24768164
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.03.017"; journalName: "Dev Cell"; date: 
            "2014-05-12- 12"; volume: "29"; issue: "3"; pages: "321-9"
COMMENT     Sequence Label: pLJM1 RagA
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    127..244
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttcccggaac cacgcccaga gcagccgcgt ccctgcgcaa acccagggct gccttggaaa
       61 aggcgcaacc ccaaccccct cgagccgcgg ccaaagtgga tctctgctgt ccctgtaata
      121 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
      181 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
      241 aaaattgtgg atgaatactg ccatttgtct cgaggtcgag aattgtcaac gcataaggag
      301 actgtgcttg gggccatcca ctctctccag cttctcaaag tgtttccggg cattgcgaat
      361 gttgatcaga gtggccgcag aagggatcga cggatctgac atgaccacca tcacgtacgt
      421 atttgaggtg aagatgtcga tgaaagcagc gaagttggaa ttcctaactt ccatgctctg
      481 gaaggaagcg gccaatttac tgcagctcag cttgaactgt ttgatgatgt tgctgatctt
      541 ctcaaaccgg tggacgtcgc gctgctcttt gcactggtag tgggaaataa ccaagaatgt
      601 agctctttcg aacagcagaa cttcatcggc ctcaatgatt tgggcaaaat tcctgaggtt
      661 catctccagc tgctgaacgt tgggaatcag ctggtagacg atgctggacc aggctttgta
      721 gagcgtctca tcccagatgg acgttcgaaa acaagcacac tccagcgggc gagacagacg
      781 cctcaggtct tcctctcgct ctttaaaaat caggtcacgc tgatcctcct gaaccagatc
      841 cattttgtgc accaggcaga agattttggc gtcaggagag ttctggagga tggcctccag
      901 acacgactgg taataatgca tgtccttttc cagttcgcgg ctctccacgt caaacacgta
      961 aatcaaaact tccacgttac ggaagatatt gtc
//
