LOCUS       sequence-164358-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single Integration Vector URA - Cloning vector for single 
            integration into the URA3 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVu
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     251..268
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     252..268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(297..313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(297..313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    314..347
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        384..727
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tgatgatatt tcataaataa tgtaattcta tatatgttaa ttaccttttt tgcgaggcat
       61 atttatggtg aaggataagt tttgaccatc aaagaaggtt aatgtggctg tggtttcagg
      121 gtccataatg cttttcaatt catctttttt ttttttgttc ttttttttga ttccggtttc
      181 tttgaaattt ttttgattcg gtaatctccg agcagaagga agaacgaagg aaggagcaca
      241 gacttagatt tgtaaaacga cggccagtgc atgcgtatcg gacgtctccc ttcatggtca
      301 tagctgtttc ctgataactt cgtataatgt atgctatacg aagttattag cttgcctcgt
      361 ccccgccggg tcacccggcg agcgacatgg aggcccagaa taccctcctt gacagtcttg
      421 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
      481 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
      541 gctcctcgct ccagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
      601 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
      661 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
      721 aacaaccatg acagtcaaca ctaataccta tagtgagaga gcagaaactc atgcctcacc
      781 agtagcacag cgattatttc gattaatgga actgaagaaa accaatttat gtgcatcaat
      841 tgacgttgat accactaagg agttccttga attaattgat aaattaggtc cttatgtatg
      901 cttaatcaag actcatattg atataatcaa tgatttttcc tatgaatcca ctattgaacc
      961 attattagaa ctttcacgta aacatcaatt tatga
//
