LOCUS       sequence-164360-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single Integration Vector URA - Cloning vector for single 
            integration into the URA3 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVu
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..92
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     93..112
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        93..111
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    120..227
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     144..160
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     144..160
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(194..210)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(195..211)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(240..258)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(626..645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgat
       61 aacttcgtat aatgtatgct atacgaagtt attaatacga ctcactatag ggcgaattgg
      121 agctccaccg cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg
      181 atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacccag cttttgttcc
      241 ctttagtgag ggttaatttc ctgaaaactg tattataagt aaatgcatgt atactaaact
      301 cacaaattag agcttcaatt taattatatc agttattacg cgggaatctc ggtcgtaatg
      361 atttctataa tgacgaaaaa aaaaaaattg gaaagaaaat gcttcatggc ctttataaaa
      421 aggaactatc caatacctcg ccagaaccaa gtaacagtat tttacggggc acaaatcaag
      481 aacaataaga caggactgta aagatggacg catttaatta attcgaaggc cgcgttgctg
      541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttga
//
