LOCUS       sequence-164362-        3963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single Integration Vector URA - Cloning vector for single 
            integration into the URA3 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVu
FEATURES             Location/Qualifiers
     source          1..3963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     254..271
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     255..271
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(300..316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(300..316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    317..350
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        387..730
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1549..1746
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    1770..1803
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1804..1823
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1804..1822
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1831..1938
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     1855..1871
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1855..1871
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1905..1921)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1906..1922)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1951..1969)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(2257..2845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2337..2356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3016..3807,3808..3876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3639..3658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3877..3940)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatgatgat atttcataaa taatgtaatt ctatatatgt taattacctt ttttgcgagg
       61 catatttatg gtgaaggata agttttgacc atcaaagaag gttaatgtgg ctgtggtttc
      121 agggtccata atgcttttca attcatcttt tttttttttg ttcttttttt tgattccggt
      181 ttctttgaaa tttttttgat tcggtaatct ccgagcagaa ggaagaacga aggaaggagc
      241 acagacttag atttgtaaaa cgacggccag tgcatgcgta tcggacgtct cccttcatgg
      301 tcatagctgt ttcctgataa cttcgtataa tgtatgctat acgaagttat tagcttgcct
      361 cgtccccgcc gggtcacccg gcgagcgaca tggaggccca gaataccctc cttgacagtc
      421 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
      481 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
      541 acggctcctc gctccagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
      601 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
      661 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
      721 ataaacaacc atgacagtca acactaatac ctatagtgag agagcagaaa ctcatgcctc
      781 accagtagca cagcgattat ttcgattaat ggaactgaag aaaaccaatt tatgtgcatc
      841 aattgacgtt gataccacta aggagttcct tgaattaatt gataaattag gtccttatgt
      901 atgcttaatc aagactcata ttgatataat caatgatttt tcctatgaat ccactattga
      961 accattatta gaactttcac gtaaacatca atttatgatt tttgaagata gaaaatttgc
     1021 tgatattggt aataccgtaa agaaacaata tattggtgga gtttataaaa ttagtagttg
     1081 ggcagatatt accaatgctc atggtgtcac tgggaatgga gtggttgaag gattaaaaca
     1141 gggagctaaa gaaaccacca ccaaccaaga gccaagaggg ttattgatgt tagctgaatt
     1201 atcatcagtg ggatcattag catacggaga atattctcaa aaaactgttg aaattgctaa
     1261 atccgataag gaatttgtta ttggatttat tgcccaacgt gatatgggtg gccaagaaga
     1321 aggatttgat tggcttatta tgacacctgg agttggatta gatgataaag gtgatggatt
     1381 aggacaacaa tatagaactg ttgatgaagt tgttagcact ggaactgata ttatcattgt
     1441 tggtagagga ttgtttggta aaggaagaga tccagatatt gaaggtaaaa ggtatagaaa
     1501 tgctggttgg aatgcttatt tgaaaaagac tggccaatta taatcagtac tgacaataaa
     1561 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
     1621 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
     1681 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
     1741 atactgctgt cgattcgata ctaacgccga taacttcgta taatgtatgc tatacgaagt
     1801 tattaatacg actcactata gggcgaattg gagctccacc gcggtggcgg ccgctctaga
     1861 actagtggat cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc
     1921 gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattt cctgaaaact
     1981 gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat
     2041 cagttattac gcgggaatct cggtcgtaat gatttctata atgacgaaaa aaaaaaaatt
     2101 ggaaagaaaa tgcttcatgg cctttataaa aaggaactat ccaatacctc gccagaacca
     2161 agtaacagta ttttacgggg cacaaatcaa gaacaataag acaggactgt aaagatggac
     2221 gcatttaatt aattcgaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3061 atttcgttca tccatagttg cctgactgcc cgtcgtgtag ataactacga tacgggaggg
     3121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3421 gttgtgaaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaagtat cgttcgaatt
     3961 aat
//
