LOCUS       sequence-164364-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil  peptide SYNZIP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pQLinkHD-SYNZIP1
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(19..33,34..39,40..56,57..63)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    71..87
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..117
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             130..150
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    163..187
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(380..479)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(496..515)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(497..515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(520..536)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(520..536)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(638..657)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(716..735)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 acaccatttg tcgagaaatc ataaaaaatt tatttgcttt gtgagcggat aacaattata
       61 atagattcaa ttgtgagcgg ataacaattt cacacagaat tcattaaaga ggagaaatta
      121 actatgaaac atcaccatca ccatcaccat ggtgctgcag gtacaagttt gtacaaaaaa
      181 gcaggctccg cggccgcccc cttcaccatg ggatccaacc tggttgcgca gctcgaaaac
      241 gaagttgcgt ctctggaaaa tgagaacgaa accctgaaga aaaagaacct gcacaaaaaa
      301 gacctgatcg cgtacctgga gaaagaaatc gcgaatctgc gtaagaaaat cgaagaatga
      361 taaaagggtg ggcgcgccga cccagctttc ttgtacaaag ttggcattat aagaaagcat
      421 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
      481 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
      541 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
      601 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
      661 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
      721 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
      781 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
      841 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
      901 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
      961 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     1021 ttcctgc
//
