LOCUS       sequence-164365-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil  peptide SYNZIP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pQLinkHD-SYNZIP1
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..74
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(267..366)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(382..399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(382..398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(391..413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      462..548
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcattactg gatctatcaa caggagtcca agctcagcta attaagctta ccactttgta
       61 caagaaagct gggtcggcgc gcccaccctt ttatcattct tcgattttct tacgcagatt
      121 cgcgatttct ttctccaggt acgcgatcag gtcttttttg tgcaggttct ttttcttcag
      181 ggtttcgttc tcattttcca gagacgcaac ttcgttttcg agctgcgcaa ccaggttgga
      241 tcccatggtg aagggggcgg ccgcggagcc tgcttttttg tacaaagttg gcattataaa
      301 aaagcattgc tcatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      361 tatttggggc ccgagcttaa gactggccgt cgttttacaa cgtcgtgact gggaaaacat
      421 ccatgctagc gttaacgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
      481 agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
      541 ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga gggtggcgg
//
