LOCUS       sequence-164367-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil peptide SYNZIP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pQLinkHD-SYNZIP2
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(26..40,41..46,47..63,64..70)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    46..62
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    78..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..124
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             137..157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    170..194
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(396..420)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      442..536
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(696..715)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 attaacaaca ccatttgtcg agaaatcata aaaaatttat ttgctttgtg agcggataac
       61 aattataata gattcaattg tgagcggata acaatttcac acagaattca ttaaagagga
      121 gaaattaact atgaaacatc accatcacca tcaccatggt gctgcaggta caagtttgta
      181 caaaaaagca ggctccgcgg ccgccccctt caccatggga tccgcgcgta acgcgtatct
      241 gcgtaagaaa atcgcacgtc tgaaaaaaga caacctgcag ctggaacgtg atgaacagaa
      301 cctggaaaaa atcatcgcga acctgcgtga cgaaatcgcg cgtctcgaaa acgaagttgc
      361 gtctcacgaa cagtgataaa agggtgggcg cgccgaccca gctttcttgt acaaagtggt
      421 aagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
      481 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
      541 gtaacaacac catttaaatg gagtggttac aaatggagtg gttaattaag gctagcttgg
      601 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
      661 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      721 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
      781 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
      841 cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
      901 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
      961 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     1021 tggcctattt
//
