LOCUS       sequence-164370-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil peptide SYNZIP3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pQLinkHD-SYNZIP3
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(18..32,33..38,39..55,56..62)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    70..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..116
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             129..149
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    162..186
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(364..388)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      410..504
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(664..683)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caccatttgt cgagaaatca taaaaaattt atttgctttg tgagcggata acaattataa
       61 tagattcaat tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaattaa
      121 ctatgaaaca tcaccatcac catcaccatg gtgctgcagg tacaagtttg tacaaaaaag
      181 caggctccgc ggccgccccc ttcaccatgg gatccaacga agttaccact ctggagaatg
      241 acgctgcgtt catcgaaaat gaaaacgctt acctggaaaa agaaatcgcg cgtctgcgta
      301 aagaaaaagc ggcgctgcgc aaccgtctgg cgcacaaaaa atgataaaag ggtgggcgcg
      361 ccgacccagc tttcttgtac aaagtggtaa gcttaattag ctgagcttgg actcctgttg
      421 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
      481 cgggcgtttt ttattggtga gaatccaagt aacaacacca tttaaatgga gtggttacaa
      541 atggagtggt taattaaggc tagcttggcg agattttcag gagctaagga agctaaaatg
      601 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
      661 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
      721 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
      781 attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag
      841 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
      901 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
      961 caagatgtgg cgtgttacgg tgaaaacctg gcctattt
//
