LOCUS       sequence-164371-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil peptide SYNZIP3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pQLinkHD-SYNZIP3
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..72
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(250..274)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(287..307)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     RBS             320..331
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    350..366
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(374..380,381..397,398..403,404..418))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    382..398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(468..486)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        554..658
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(877..896)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cattactgga tctatcaaca ggagtccaag ctcagctaat taagcttacc actttgtaca
       61 agaaagctgg gtcggcgcgc ccaccctttt atcatttttt gtgcgccaga cggttgcgca
      121 gcgccgcttt ttctttacgc agacgcgcga tttctttttc caggtaagcg ttttcatttt
      181 cgatgaacgc agcgtcattc tccagagtgg taacttcgtt ggatcccatg gtgaaggggg
      241 cggccgcgga gcctgctttt ttgtacaaac ttgtacctgc agcaccatgg tgatggtgat
      301 ggtgatgttt catagttaat ttctcctctt taatgaattc tgtgtgaaat tgttatccgc
      361 tcacaattga atctattata attgttatcc gctcacaaag caaataaatt ttttatgatt
      421 tctcgacaaa tggtgttgtt aattaagctc gaggtgaaga cgaaagggcc tcgtgatacg
      481 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      541 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      601 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      661 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      721 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      781 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      841 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      901 tat
//
