LOCUS       sequence-164372-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil peptide SYNZIP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pQLinkHD-SYNZIP4
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(18..32,33..38,39..55,56..62)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    70..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..116
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             129..149
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    162..186
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(400..424)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      446..540
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(700..719)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caccatttgt cgagaaatca taaaaaattt atttgctttg tgagcggata acaattataa
       61 tagattcaat tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaattaa
      121 ctatgaaaca tcaccatcac catcaccatg gtgctgcagg tacaagtttg tacaaaaaag
      181 caggctccgc ggccgccccc ttcaccatgg gatcccagaa agttgcggaa ctcaaaaacc
      241 gtgttgcggt taaactgaat cgtaacgaac agctgaaaaa caaagttgaa gagctgaaga
      301 accgtaacgc ttacctcaag aacgaactgg cgaccctgga gaacgaggtt gcgcgtctgg
      361 aaaacgacgt tgcagaatga taaaagggtg ggcgcgccga cccagctttc ttgtacaaag
      421 tggtaagctt aattagctga gcttggactc ctgttgatag atccagtaat gacctcagaa
      481 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat
      541 ccaagtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taaggctagc
      601 ttggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      661 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      721 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      781 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
      841 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
      901 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
      961 cacgacgatt tccggcagtt tctacacata tattcg
//
