LOCUS       sequence-164374-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil peptide SYNZIP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pQLinkHD-SYNZIP4
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(279..303)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(316..336)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     RBS             349..360
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    379..395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(403..409,410..426,427..432,433..447))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    411..427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(497..515)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        583..687
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatctatca acaggagtcc aagctcagct aattaagctt accactttgt acaagaaagc
       61 tgggtcggcg cgcccaccct tttatcattc tgcaacgtcg ttttccagac gcgcaacctc
      121 gttctccagg gtcgccagtt cgttcttgag gtaagcgtta cggttcttca gctcttcaac
      181 tttgtttttc agctgttcgt tacgattcag tttaaccgca acacggtttt tgagttccgc
      241 aactttctgg gatcccatgg tgaagggggc ggccgcggag cctgcttttt tgtacaaact
      301 tgtacctgca gcaccatggt gatggtgatg gtgatgtttc atagttaatt tctcctcttt
      361 aatgaattct gtgtgaaatt gttatccgct cacaattgaa tctattataa ttgttatccg
      421 ctcacaaagc aaataaattt tttatgattt ctcgacaaat ggtgttgtta attaagctcg
      481 aggtgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      541 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      601 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      661 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      721 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      781 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      841 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cactttt
//
