LOCUS       sequence-164378-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9-carrying vector with kanMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Stovicek V, Borodina I, Forster J
  TITLE     CRISPR-Cas system enables fast and simple genome editing of 
            industrial Saccharomyces cerevisiae strains.
  JOURNAL   Metab Eng Commun. 2015 Mar 20;2:13-22. doi: 
            10.1016/j.meteno.2015.03.001. eCollection 2015 Dec.
  PUBMED    34150504
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2015-03-20"; volume: "2:13-22. doi"; pages: " 
            10.1016/j.meteno.2015.03.001. eCollection 2015 Dec"
COMMENT     Sequence Label: pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    592..625
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taattataat ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag
       61 aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc
      121 aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc
      181 atcttgtctg tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt
      241 taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta
      301 cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct
      361 tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca
      421 gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca
      481 agcgatccgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
      541 cgagatcgcg tcagctgaag cttcgtacgc tgcaggtcga caacccttaa tataacttcg
      601 tataatgtat gctatacgaa gttattaggt ctagagatct gtttagcttg cctcgtcccc
      661 gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt
      721 gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt
      781 ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc
      841 ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca
      901 cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca
      961 tatactt
//
