LOCUS       sequence-164383-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil peptide SYNZIP6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pQLinkHD-SYNZIP6
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    52..76
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(290..314)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(327..347)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     RBS             360..371
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    390..406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(414..420,421..437,438..443,444..458))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    422..438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(508..526)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        594..698
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aggtcattac tggatctatc aacaggagtc caagctcagc taattaagct taccactttg
       61 tacaagaaag ctgggtcggc gcgcccaccc ttttatcaac gcgcaacgtc acgttccaga
      121 tttgcgatgt ccttttcgag attcgcaatg tctttctcca ggttcgcatt gtcgttttcc
      181 agacgcgcca cgatgttctc cagcttcgcg ttttctttca gtttgtacgc aacacggttt
      241 ttcagctgcg caactttttg ggatcccatg gtgaaggggg cggccgcgga gcctgctttt
      301 ttgtacaaac ttgtacctgc agcaccatgg tgatggtgat ggtgatgttt catagttaat
      361 ttctcctctt taatgaattc tgtgtgaaat tgttatccgc tcacaattga atctattata
      421 attgttatcc gctcacaaag caaataaatt ttttatgatt tctcgacaaa tggtgttgtt
      481 aattaagctc gaggtgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
      541 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      661 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      781 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      841 atctcaacag cggtaagatc cttgagagtt ttcgccccga a
//
