LOCUS       sequence-164407-        1164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Venus tagged Cdc-42 sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   O'Neill PR, Kalyanaraman V, Gautam N
  TITLE     Subcellular optogenetic activation of Cdc42 controls local and 
            distal signaling to drive immune cell migration.
  JOURNAL   Mol Biol Cell. 2016 May 1;27(9):1442-50. doi: 
            10.1091/mbc.E15-12-0832. Epub 2016 Mar 3.
  PUBMED    26941336
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-12-0832"; journalName: "Mol Biol Cell"; date: 
            "2016-05-1- 1"; volume: "27"; issue: "9"; pages: "1442-50"
COMMENT     Sequence Label: Venus wGBD
FEATURES             Location/Qualifiers
     source          1..1164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(302..321)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             461..712
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     649..670
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctgncctag nnnttnnnnn taagctatgt nnnngnagnn ngtnnncnnn aggnnnnntc
       61 ctgtcnannt ggacgngaac gtaanncana gtcagnggtg tngnnnnngn nnngatgcac
      121 tacgcnagct gaacctgaag ctgatctgca caccgcagct gccgtgcctg nccaccctcg
      181 tgaccacnng ggctacgnct gcagtgcttc gcccgctacc ccgaccncca tgaagcagca
      241 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      301 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      361 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      421 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
      481 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
      541 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      601 gagctaccag tccaaactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      661 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggtacccc
      721 tagcccagct gataagaaac gctcagggaa gaagaagatc agcaaagctg atattggtgc
      781 acccagtgga ttcaagcatg tcagccacgt ggggtgggac ccccagaatg gatttgacgt
      841 gaacaacctc gacccagatc tgcggagtct gttctccagg gcaggaatca gcgaggccca
      901 gctcaccgac gccgagacct ctaaacttat ctacgacttc attgaggacc agggtgggct
      961 ggaggctgtg cggcaggaga tgaggcgcca ggagttctag agaattccga tttccaaagc
     1021 gggaaaggaa atcaaggagt acgtggaggc ccaagcagga aacgatcctt ttctcaaagg
     1081 catccctgag gacaagaatc ccttcaagga gaaaggtggc tgtctgataa gctgactcga
     1141 gtctagaang ggcccccntn ncnc
//
