LOCUS       sequence-164408-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  full length Nedd4L with His Xpress tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Itani OA, Stokes JB, Thomas CP
  TITLE     Nedd4-2 isoforms differentially associate with ENaC and regulate its
            activity.
  JOURNAL   Am J Physiol Renal Physiol. 2005 Aug;289(2):F334-46. Epub 2005 Apr 
            5.
  PUBMED    15814530
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Renal Physiol."; date: "2005-08"; volume: "289(2)"; pages: 
            "F334-46. Epub 2005 Apr 5"
COMMENT     Sequence Label: hNedd4-2
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    108..270
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             283..300
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             304..336
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(340..348,349..363)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttagc gcagaggctt
      121 ggggcagccg agcggcagcc aggccccggc ccgggcctcg gttccagaag ggagaggagc
      181 ccgccaaggc gcgcaagaga gcgggctgcc tcgcagtccg agccggagag ggagcgcgag
      241 ccgcgccggc cccggacggc ctccgaaacc atggggggtt ctcatcatca tcatcatcat
      301 ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga cgatgacgat
      361 aaggtacgcg ccggctccat ggcgaccggg ctcggggagc cggtctatgg actttccgaa
      421 gacgagggag agtcccgtat tctcagagta aaagttgttt ctggaattga tctcgccaaa
      481 aaggacatct ttggagccag tgatccgtat gtgaaacttt cattgtacgt agcggatgag
      541 aatagagaac ttgctttggt ccagacaaaa acaattaaaa agacactgaa cccaaaatgg
      601 aatgaagaat tttatttcag ggtaaaccca tctaatcaca gactcctatt tgaagtattt
      661 gacgaaaata gactgacacg agacgacttc ctgggccagg tggacgtgcc ccttagtcac
      721 cttccgacag aagatccaac catggagcga ccctatacat ttaaggactt tctcctcaga
      781 ccaagaagtc ataagtctcg agttaaggga tttttgcgat tgaaaatggc ctatatgcca
      841 aaaaatggag gtcaagatga agaaaacagt gaccagaggg atgacatgga gcatggatgg
      901 gaagttgttg actcaaatga ctcggcttct cagcaccaag aggaacttcc tcctcctcct
      961 ctgcctcccg ggtgggaaga aaaagtggac aatttaggcc gaact
//
