LOCUS       sequence-164417-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of doxycycline-inducible constitutively active
            Rac1 and co-expression of Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   MacKay JL, Kumar S
  TITLE     Simultaneous and independent tuning of RhoA and Rac1 activity with 
            orthogonally inducible promoters.
  JOURNAL   Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d.
  PUBMED    25044255
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Integr Biol (Camb)"; date: "2014-09"; volume: "6"; issue: "9"; 
            pages: "885-94"
COMMENT     Sequence Label: pSLIK CA Rac1
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        140..454
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    148..166
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    184..202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    219..237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    255..273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    326..344
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    362..380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     427..451
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
       61 tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt aaaggaacca
      121 attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
      181 tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg atagagaacg
      241 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat gtcgagttta
      361 ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg aggcctatat
      421 aagcagagct cgtttagtga accgtcagat cgccactagt cgactggatc cggtaccgaa
      481 ttcatgcagg ccatcaagtg tgtggtggtg ggagacggag ctgtaggtaa aacttgccta
      541 ctgatcagtt acacaaccaa tgcatttcct ggagaatata tccctactgt ctttgacaat
      601 tattctgcca atgttatggt agatggaaaa ccggtgaatc tgggcttatg ggatacagct
      661 ggactagaag attatgacag attacgcccc ctatcctatc cgcaaacaga tgtgttctta
      721 atttgctttt cccttgtgag tcctgcatca tttgaaaatg tccgtgcaaa gtggtatcct
      781 gaggtgcggc accactgtcc caacactccc atcatcctag tgggaactaa acttgatctt
      841 agggatgata aagacacgat cgagaaactg aaggagaaga agctgactcc catcacctat
      901 ccgcagggtc tagccatggc taaggagatt ggtgctgtaa aatacctgga gtgctcggcg
      961 ctcacacagc gaggcctcaa gacagtgttt gacgaagcga tccgagcagt cctctgcccg
     1021 cctcccg
//
