LOCUS       sequence-164419-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR - FLIP targeting vector for mouse beta-catenin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, 
            Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK
  TITLE     One-step generation of conditional and reversible gene knockouts.
  JOURNAL   Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
  PUBMED    28135257
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156."
COMMENT     Sequence Label: pUC118-mCtnnb1-FLIP-Puro
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             288..323
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgaattcga gctcggtacc cggggatcct
      241 ctagagtcgt gacagctgac ctgatggagt tggacatggc catggagccg gacagaaaag
      301 ctgctgtcag ccactggcag cagcagtctt acttggattc tggaatccat tctggtgcca
      361 ccaccacagc tccttccctg agtggcaagg gcaaccctga ggaagaagat gttgacacct
      421 cccaagtcct ttatgaatgg gagcaaggct tttcccagtc cttcacgcaa gagcaagtag
      481 ctggtaaagc atttgtgttt ggaagctagc attaagtttc ttgacagggc tgtgtgacag
      541 ctcagccaca gcacaagtgg gttgaaggaa gggcggaggg tagcggagtc ccacatgcct
      601 agtgagtgtt ggatttacct ttttcagata ttgacgggca gtatgcaatg actagggctc
      661 agagggtccg agctgccatg ttccctgaga cgctagatga gggcatgcag atcccatcca
      721 cgcagtttga cgctgctcat cccactaatg tccagcgctt ggctgaacca tcacagatgt
      781 tgaaacatgc agttgtcaat ttgattaact atcaggatga cgcggaactt gccacacgtg
      841 caattcctga gctgacaaaa ctgctaaacg atgaggacca ggtaagcagt gatgtaactt
      901 gcttttcagt ttactttgag g
//
