LOCUS       sequence-164421-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, 
            Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK
  TITLE     One-step generation of conditional and reversible gene knockouts.
  JOURNAL   Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
  PUBMED    28135257
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156."
COMMENT     Sequence Label: pUC118-mCtnnb1-FLIP-FlpE-Puro
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     167..189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             287..322
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg accatgatta cgaattcgag ctcggtaccc ggggatcctc
      241 tagagtcgtg acagctgacc tgatggagtt ggacatggcc atggagccgg acagaaaagc
      301 tgctgtcagc cactggcagc agcagtctta cttggattct ggaatccatt ctggtgccac
      361 caccacagct ccttccctga gtggcaaggg caaccctgag gaagaagatg ttgacacctc
      421 ccaagtcctt tatgaatggg agcaaggctt ttcccagtcc ttcacgcaag agcaagtagc
      481 tggtaaagca tttgtgtttg gaagctagca ttaagtttct tgacagggct gtgtgacagc
      541 tcagccacag cacaagtggg ttgaaggaag ggcggagggt agcggagtcc cacatgccta
      601 gtgagtgttg gatttacctt tttcagatat tgacgggcag tatgcaatga ctagggctca
      661 gagggtccga gctgccatgt tccctgagac gctagatgag ggcatgcaga tcccatccac
      721 gcagtttgac gctgctcatc ccactaatgt ccagcgcttg gctgaaccat cacagatgtt
      781 gaaacatgca gttgtcaatt tgattaacta tcaggatgac gcggaacttg ccacacgtgc
      841 aattcctgag ctgacaaaac tgctaaacga tgaggaccag gtaagcagtg atgtaacttg
      901 cttttcagt
//
