LOCUS       sequence-164422-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of doxycycline-inducible dominant negative 
            RhoA and co-expression of Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   MacKay JL, Kumar S
  TITLE     Simultaneous and independent tuning of RhoA and Rac1 activity with 
            orthogonally inducible promoters.
  JOURNAL   Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d.
  PUBMED    25044255
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Integr Biol (Camb)"; date: "2014-09"; volume: "6"; issue: "9"; 
            pages: "885-94"
COMMENT     Sequence Label: pSLIK DN RhoA
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    154..178
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(257..781,782..784,785..838))
                     /codon_start=1
                     /product="dominant negative mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (T19N)
                     /translation="MAAIRKKLVIVGDGACGKNCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
ORIGIN
        1 ggcggaagga tcaggacgct cgctgcgccc ttcgtctgac gtggcagcgc tcgccgtgag
       61 gaggggggcg cccgcgggag gcgccaaaac ccggcgcgga ggccagatct tgggtgggtt
      121 aattaaccat ctttatggct cgagatgtta tcaaccactt tgtacaagaa agctgggtct
      181 agatatctcg agcggccgcc agtgtgatgg atatctgcag aattcaccgc ataagggctg
      241 tgcttccagc aaggtttcac aagacaaggc acccagattt tttcttccca cgtctagctt
      301 gcagagcagc tctcgtagcc atttcaaaaa cctctctcac tccatctttg gtctttgctg
      361 aacactccat gtacccaaaa gcgccaatcc tgtttgccat atctctgcct tcttcaggtt
      421 tcaccggctc ctgcttcatc ttggctagct cccgccttgt gtgctcatca ttccgaagat
      481 ccttcttatt cccaaccagg atgatgggca cgttgggaca gaaatgcttg acttctgggg
      541 tccacttttc tgggatgttt tctaaactat cagggctgtc gatggaaaaa cacatcagta
      601 taacatcggt atctgggtag gagaggggcc tcaggcgatc ataatcttcc tgcccagctg
      661 tgtcccacaa agccaactct acctgctttc catccacctc gatatctgcc acatagttct
      721 caaacactgt gggcacatac acctctggga actggtcctt gctgaagact atgagcaagc
      781 agttctttcc acaggctcca tcaccaacaa tcaccagttt cttccggatg gcagccatgg
      841 gatcccaggt ccctcctcgg agatcagctt ctgctccatg ggaaccggaa t
//
