LOCUS       sequence-164424-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of doxycycline-inducible constitutively active
            ROCK2 and co-expression of Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Wong SY, Ulrich TA, Deleyrolle LP, MacKay JL, Lin JM, Martuscello 
            RT, Jundi MA, Reynolds BA, Kumar S
  TITLE     Constitutive activation of myosin-dependent contractility sensitizes
            glioma tumor-initiating cells to mechanical inputs and reduces 
            tissue invasion.
  JOURNAL   Cancer Res. 2015 Mar 15;75(6):1113-22. doi: 
            10.1158/0008-5472.CAN-13-3426. Epub 2015 Jan 29.
  PUBMED    25634210
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-13-3426"; journalName: "Cancer Res"; date: 
            "2015-03-15- 15"; volume: "75"; issue: "6"; pages: "1113-22"
COMMENT     Sequence Label: pSLIK CA ROCK2
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        140..454
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    148..166
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    184..202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    219..237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    255..273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    326..344
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    362..380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     427..451
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
       61 tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt aaaggaacca
      121 attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
      181 tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg atagagaacg
      241 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat gtcgagttta
      361 ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg aggcctatat
      421 aagcagagct cgtttagtga accgtcagat cgccactagt cgactggatc cggtaccgaa
      481 ttcctgcagc ccgggggatc cgggtaccat gggccgacgg ggaagatgcc cggcgccccc
      541 gaggccgtgt cgggggacgg cgcgggcgcg agccgccaga ggaagctgga agcgctgatc
      601 cgagaccctc gttcgcccat caacgtggag agcttgctgg atggcttaaa ttctttggtc
      661 cttgatttgg attttcctgc tttgaggaaa aacaaaaata tagataattt cttaaataga
      721 tatgagaaaa ttgtgaaaaa aattagaggt ttacagatga aggcagaaga ctacgatgtt
      781 gtaaaagtta tcggaagagg tgcttttggt gaagtccagt tggttcgtca taaggcatca
      841 cagaaagttt atgctatgaa gcttcttagt aagtttgaaa tgataaaaag atcagattct
      901 gcttttttct gggaggaaag agatattatg gcctttgcca acagtccctg ggtggttcag
      961 ctcttttgtg cctttcaaga tgataggtat ctgtacatgg taatggagta catgcctggt
     1021 ggagaccttg taaacct
//
