LOCUS       sequence-164429-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of doxycycline-inducible constitutively active
            ROCK2 and co-expression of Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Wong SY, Ulrich TA, Deleyrolle LP, MacKay JL, Lin JM, Martuscello 
            RT, Jundi MA, Reynolds BA, Kumar S
  TITLE     Constitutive activation of myosin-dependent contractility sensitizes
            glioma tumor-initiating cells to mechanical inputs and reduces 
            tissue invasion.
  JOURNAL   Cancer Res. 2015 Mar 15;75(6):1113-22. doi: 
            10.1158/0008-5472.CAN-13-3426. Epub 2015 Jan 29.
  PUBMED    25634210
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-13-3426"; journalName: "Cancer Res"; date: 
            "2015-03-15- 15"; volume: "75"; issue: "6"; pages: "1113-22"
COMMENT     Sequence Label: pSLIK CA ROCK2
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    161..185
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     216..232
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     216..232
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 acgtccgggc ggaaggatca ggacgctcgc tgcgcccttc gtctgacgtg gcagcgctcg
       61 ccgtgaggag gggggcgccc gcgggaggcg ccaaaacccg gcgcggaggc cagatcttgg
      121 gtgggttaat taaccatctt tatggctcga gatgttatca accactttgt acaagaaagc
      181 tgggtctaga tatctcgagt gcggccgcct cggcctctag aactagtgga tccgggtacc
      241 atgggccgac aatgataact actatgtgta aaaatagtgc actaattttc ttctgcaaag
      301 caaatttgca tcctaagagc tggcaaatac tttttttttt gttttaaata caggaaaggg
      361 tggatatctg agagttctga tttctttttt tcaaatcttc aagctgatct tttaaactgt
      421 taacatcatt ctccaaattt cgcttcttgt ctgcttcatg atcagctttc cgctgatatt
      481 ctgcattttt gtgctgaaga agtgcttttt ctctttctaa ttgtcttaat gttgattcca
      541 catttttcct taaggtaatc tcttcttcta actcctttgc cactttctct aagcgagtat
      601 taacagactt gcacttctgt tctagttcct ctttggcctg aatctcagtg ctaaggtgtt
      661 cttctaatgt gtacagtttt ttctgaatct cttgactctc ttcattcttc cttgattgaa
      721 ttgagtcatt ttctttacaa gatggagagt cacttagtag caaattttct ctgtagtagg
      781 taaatcctat aaaaggtagc tgatttccca caaaagcctt gggaattggg aaggtttcta
      841 catctccttt atcatcttca atgtcatcaa aattgctgct gtctatgtca ctgctgagtt
      901 caggtaccac aggagctgca gtctctctta tgttatccca attccactga tcattcttaa
      961 agaaaggat
//
