LOCUS       sequence-164437-         520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Role of Rac selective GEF domain from Tiam1 in immune cell 
            migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 520)
  AUTHORS   O'Neill PR, Kalyanaraman V, Gautam N
  TITLE     Subcellular optogenetic activation of Cdc42 controls local and 
            distal signaling to drive immune cell migration.
  JOURNAL   Mol Biol Cell. 2016 May 1;27(9):1442-50. doi: 
            10.1091/mbc.E15-12-0832. Epub 2016 Mar 3.
  PUBMED    26941336
REFERENCE   2  (bases 1 to 520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-12-0832"; journalName: "Mol Biol Cell"; date: 
            "2016-05-1- 1"; volume: "27"; issue: "9"; pages: "1442-50"
COMMENT     Sequence Label: mTiam1-mcherry-sspB in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(29..148,149..151,152..358))
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
ORIGIN
        1 gnnngnntac ggggactcta gattaaccaa tattcagctc gtcatagatt tcttctggtt
       61 cgaacatcac accatcgccg ttctcgcgag cgtaaatggc cagagcggca cccatcggga
      121 tatacagttc acgagacacg cccttaaact gggcgttgaa ctggataaaa tcatttgtca
      181 gttgcaggtt gccggtcgca cttgcagaca gattcagcac gatctgaccg tctttcacat
      241 actccacggg cacgttcacg cccaggtatg tggcatccac caccagatat ggggtaaagc
      301 tgttatcaac cagccaatcg taatattcac gcagcagctt agggcgtttc ggggagctga
      361 attcctgcag ggtcaggccg ctcagatcaa tgccgaattc gttcgaagct tgagctcgag
      421 atctgagtcc ggacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
      481 gttcgtactg ttccacgatg gtgtagtcct cgttgtgggg
//
