LOCUS       sequence-164447-         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR HDAC3 dual gRNA targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  TITLE     Chen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-L3US2-RFP-HDAC3
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..110
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_RNA        complement(151..226)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(245..493)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(303..322)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(473..493)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_RNA        complement(526..601)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gtagcgcttc tagaaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttt ttataatgcc aactttgtat aataaagttg tctagaggtc
      121 tggaagcagt atgtggggta ccaaaaaaaa gcaccgactc ggtgccactt tttcaagttg
      181 ataacggact agccttattt taacttgcta tttctagctc taaaacatcg cctggcattg
      241 acccggtgtt tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag
      301 ttacggtaag catatgatag tccattttaa aacataattt taaaactgca aactacccaa
      361 gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat
      421 taattccaat tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg
      481 gaaataggcc ctccctagtc cttatcttgc tcgttggtca aaaaagcacc gactcggtgc
      541 cactttttca agttgataac ggactagcct tattttaact tgctatttct agctctaaaa
      601 cgtgtaacgc gagcagaacg gtgtttcgtc ctttccacaa gatata
//
