LOCUS       sequence-164448-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR HDAC3 dual gRNA targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  TITLE     Chen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-L3US2-RFP-HDAC3
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             107..838
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             854..910
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(911..930)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
       61 gggtttgccg ccagaacaca ggtgtcgtga cgcgggatcc gccaccatgg tgtctaaggg
      121 cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa
      181 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
      241 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag
      301 cttcatgtac ggcagcagaa ccttcatcaa ccacacccag ggcatccccg atttctttaa
      361 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
      421 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
      481 cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
      541 ggccaacacc gagatgctgt accccgctga cggcggcctg gaaggcagaa ccgacatggc
      601 cctgaagctc gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa
      661 gaaacccgct aagaacctca agatgcccgg cgtctactat gtggaccaca gactggaaag
      721 aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata
      781 ctgcgacctc cctagcaaac tggggcacaa acttaatggc atggacgagc tgtacaaggc
      841 tagcggcagc ggcgccacca acttcagcct gctgaagcag gccggcgacg tggaggagaa
      901 ccccggcccc atgaccgagt acaagcccac g
//
