LOCUS       sequence-164452-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rOpto-mFGFR1 (light-activated receptor tyrosine kinase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Reichhart E, Ingles-Prieto A, Tichy AM, McKenzie C, Janovjak H
  TITLE     A Phytochrome Sensory Domain Permits Receptor Activation by Red 
            Light.
  JOURNAL   Angew Chem Int Ed Engl. 2016 May 17;55(21):6339-42. doi: 
            10.1002/anie.201601736. Epub 2016 Apr 21.
  PUBMED    27101018
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/anie.201601736"; journalName: "Angew Chem Int Ed Engl"; 
            date: "2016-05-17- 17"; volume: "55"; issue: "21"; pages: "6339-42"
COMMENT     Sequence Label: rOpto-mFGFR1_333
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      125..134
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             131..172
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaacgg gccctctaga ctcgagcggc
      121 cgcggccacc atggggagta gcaagagcaa gcctaaggac cccagccagc gcctcgacat
      181 gaagagcggc accaagaaga gcgacttcca tagccagatg gctgtgcaca agctggccaa
      241 gagcatccct ctgcgcagac aggtaacagt gtcagctgac tccagtgcat ccatgaactc
      301 tggggttctc ctggttcggc cctcacggct ctcctccagc gggaccccca tgctggctgg
      361 agtctccgaa tatgagctcc ctgaggatcc ccgctgggag ctgccacgag acagactggt
      421 cttaggcaaa ccacttggcg agggctgctt cgggcaggtg gtgttggctg aggccatcgg
      481 gctggataag gacaaaccca accgtgtgac caaagtggcc gtgaagatgt tgaagtccga
      541 cgcaacggag aaggacctgt cggatctgat ctcggagatg gagatgatga aaatgattgg
      601 gaagcacaag aatatcatca accttctggg agcgtgcaca caggatggtc ctctttatgt
      661 cattgtggag tacgcctcca aaggcaatct ccgggagtat ctacaggccc ggaggcctcc
      721 tgggctggag tactgctata accccagcca caaccccgag gaacagctgt cttccaaaga
      781 tctggtatcc tgtgcctatc aggtggctcg g
//
