LOCUS       sequence-164457-        2234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Role of Cdc-42 selective GEF domain from ITSN in immune cell 
            migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   O'Neill PR, Kalyanaraman V, Gautam N
  TITLE     Subcellular optogenetic activation of Cdc42 controls local and 
            distal signaling to drive immune cell migration.
  JOURNAL   Mol Biol Cell. 2016 May 1;27(9):1442-50. doi: 
            10.1091/mbc.E15-12-0832. Epub 2016 Mar 3.
  PUBMED    26941336
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-12-0832"; journalName: "Mol Biol Cell"; date: 
            "2016-05-1- 1"; volume: "27"; issue: "9"; pages: "1442-50"
COMMENT     Sequence Label: ITSN-mcherry-sspB
FEATURES             Location/Qualifiers
     source          1..2234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1110..1817
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1255..1273)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1523..1542
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(1893..2099,2100..2102,2103..2222)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
ORIGIN
        1 ggtaccgcgg gcccgccacc atgttgaccc caactgaaag aaagcgacaa ggatacatcc
       61 acgagctcat tgtcaccgag gagaactatg tgaatgacct gcagctggtc acagagattt
      121 ttcaaaaacc cctgatggag tctgagctgc tgacagaaaa agaggttgct atgatttttg
      181 tgaactggaa ggagctgatt atgtgtaata tcaaactact aaaagcgctg agagtccgca
      241 agaagatgtc cggggagaag atgcctgtga agatgattgg agacatcctg agcgcacagc
      301 tgccgcacat gcagccctac atccgcttct gcagccgcca gctcaacggg gctgccctga
      361 tccagcagaa gacggacgag gccccagact tcaaggagtt cgtcaaaaga ttggcaatgg
      421 atcctcggtg taaagggatg ccactctcta gttttatact gaagcctatg caacgggtaa
      481 caagataccc actgatcatt aaaaatatcc tggaaaacac ccctgaaaac cacccggacc
      541 acagccactt gaagcacgcc ctggagaagg cggaagagct ctgttcccag gtgaacgaag
      601 gggtgcggga gaaggagaac tctgaccggc tggagtggat ccaggcccac gtgcagtgtg
      661 aaggcctgtc tgagcaactt gtgttcaatt cagtgaccaa ttgcttgggg ccgcgcaaat
      721 ttctgcacag tgggaagctc tacaaggcca agagcaacaa ggagctgtat ggcttccttt
      781 tcaacgactt cctcctgctg actcagatca cgaagccttt ggggtcttct ggcaccgaca
      841 aagtcttcag ccccaaatca aacctgcagt ataaaatgta taaaacacct attttcctaa
      901 atgaggttct agtaaaatta cccaccgacc cttctggaga cgagcccatc ttccacatct
      961 cccacattga ccgcgtctat actctccgag cagaaagcat aaatgaaagg actgcctggg
     1021 tgcagaaaat caaagctgct tctgaactct acatagagac tgagaaaaag aaggaattca
     1081 gtgctggatc cggtagtgga agtggttcca tggtgagcaa gggcgaggag gataacatgg
     1141 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1201 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1261 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1321 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1381 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1441 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1501 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1561 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1621 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1681 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     1741 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     1801 tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaac gaattcggca
     1861 ttgatctgag cggcctgacc ctgcaggaat tcagctcccc gaaacgccct aagctgctgc
     1921 gtgaatatta cgattggctg gttgataaca gctttacccc atatctggtg gtggatgcca
     1981 catacctggg cgtgaacgtg cccgtggagt atgtgaaaga cggtcagatc gtgctgaatc
     2041 tgtctgcaag tgcgaccggc aacctgcaac tgacaaatga ttttatccag ttcaacgccc
     2101 agtttaaggg cgtgtctcgt gaactgtata tcccgatggg tgccgctctg gccatttacg
     2161 ctcgcgagaa cggcgatggt gtgatgttcg aaccagaaga aatctatgac gagctgaata
     2221 ttggttaatc taga
//
