LOCUS       sequence-164468-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed N-term tagged UnaG on Sec61B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Park JS, Nam E, Lee HK, Lim MH, Rhee HW
  TITLE     In Cellulo Mapping of Subcellular Localized Bilirubin.
  JOURNAL   ACS Chem Biol. 2016 May 27.
  PUBMED    27232847
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2016 May 27."
COMMENT     Sequence Label: pCDNA3_UnaG-Flag-Sec61B
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             435..458
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             465..755
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     promoter        complement(783..801)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(784..801)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(821..838)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    827..938
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccaagcatag cggcgcacca tggttgaaat ttgttggtac gtggaaaata gcggattctc
       61 ataattttgg cgaatatctg aaggctatcg gggcgcctaa agagctgagt gatggaggcg
      121 atgccacgac tccaactctg tatatttcac agaaggacgg tgataaaatg accgttaaga
      181 tcgagaatgg cccgcccacc ttcctggata cacaggtaaa gtttaaactt ggtgaagaat
      241 ttgatgagtt cccgagcgac agacgcaaag gtgttaaatc cgtagtcaac ttggtcggag
      301 aaaaactcgt atatgtccaa aaatgggatg gcaaagagac aacctacgtg cgtgaaatta
      361 aagacgggaa actggttgtg acgctgacaa tgggcgacgt ggtggcagtg cgctcgtacc
      421 ggcgtgcaac cgaagactac aaggatgacg acgataagga attcatgcct ggtccgaccc
      481 ccagtggcac taacgtggga tcctcagggc gctctcccag caaagcagtg gccgcccggg
      541 cggcgggatc cactgtccgg cagaggaaaa atgccagctg tgggacaagg agtgcaggcc
      601 gcacaacctc ggcaggcacc ggggggatgt ggcgattcta cacagaagat tcacctgggc
      661 tcaaagttgg ccctgttcca gtattggtta tgagtcttct gttcatcgct tctgtattta
      721 tgttgcacat ttggggcaag tacactcgtt cgtgactcga gcatgcatct agagggccct
      781 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
      841 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
      901 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
      961 ctatctgggg gggggggggg tgggggcagg accaccaagg gggaaggaat tgggaaaaac
     1021 aaaacccggg cgggccgggg gaggcggggg ggccccaagg gctctcaagg ggggaaaa
//
