LOCUS       sequence-164481-        3446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FAD domain of P450 BM3 variant (C773S/C810S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pBsaFa
FEATURES             Location/Qualifiers
     source          1..3446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        90..192
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             193..852
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(259..278)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    1060..1081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1096..1101,1102..1119,1120..1126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1134..1150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1139..1161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1158..1174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1158..1174
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2463..2480)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2463..2479)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2472..2494)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2634..2651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2805..3393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2885..2904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
       61 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
      121 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      181 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      241 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      301 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      361 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      421 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      481 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      541 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      601 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      661 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      721 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
      781 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      841 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct
      901 gaataagtga taataagcgg atgaatggca gaaattcagc ttggcccagt gccaagctcc
      961 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1021 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1081 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1141 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcg agctcgggtc
     1201 tcgggaggta gcatgccgct tgcgaaaatg cacggtgcgt tttcaacgaa cgtcgtagca
     1261 agcaaagaac ttcaacagcc aggcagtgca cgaagcacgc gacatcttga aattgaactt
     1321 ccaaaagaag cttcttatca agaaggagat catttaggtg ttattcctcg caactatgaa
     1381 ggaatagtaa accgtgtaac agcaaggttc ggcctagatg catcacagca aatccgtctg
     1441 gaagcagaag aagaaaaatt agctcatttg ccactcgcta aaacagtatc cgtagaagag
     1501 cttctgcaat acgtggagct tcaagatcct gttacgcgca cgcagcttcg cgcaatggct
     1561 gctaaaacgg tcagcccgcc gcataaagta gagcttgaag ccttgcttga aaagcaagcc
     1621 tacaaagaac aagtgctggc aaaacgttta acaatgcttg aactgcttga aaaatacccg
     1681 gcgagcgaaa tgaaattcag cgaatttatc gcccttctgc caagcatacg cccgcgctat
     1741 tactcgattt cttcatcacc tcgtgtcgat gaaaaacaag caagcatcac ggtcagcgtt
     1801 gtctcaggag aagcgtggag cggatatgga gaatataaag gaattgcgtc gaactatctt
     1861 gccgagctgc aagaaggaga tacgattacg tgctttattt ccacaccgca gtcagaattt
     1921 acgctgccaa aagaccctga aacgccgctt atcatggtcg gaccgggaac aggcgtcgcg
     1981 ccgtttagag gctttgtgca ggcgcgcaaa cagctaaaag aacaaggaca gtcacttgga
     2041 gaagcacatt tatacttcgg ctgccgttca cctcatgaag actatctgta tcaagaagag
     2101 cttgaaaacg cccaaagcga aggcatcatt acgcttcata ccgctttttc tcgcatgcca
     2161 aatcagccga aaacatacgt tcagcacgta atggaacaag acggcaagaa attgattgaa
     2221 cttcttgatc aaggagcgca cttctatatt tgcggagacg gaagccaaat ggcacctgcc
     2281 gttgaagcaa cgcttatgaa aagctatgct gacgttcacc aagtgagtga agcagacgct
     2341 cgcttatggc tgcagcagct agaagaaaaa ggccgatacg caaaagacgt gtgggctggg
     2401 taaggatctg agaccgtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg
     2461 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2521 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2581 cgcccttccc aacagttgcg cagcctgaat ggcgaatgag cttcttccgc ttcctcgctc
     2641 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2701 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2761 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2821 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2881 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2941 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     3001 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3061 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3121 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3181 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3241 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3301 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3361 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3421 tctgacgctc agtggaactc cgtcga
//
