LOCUS       sequence-164489-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single Integration Vector LEU - Cloning vector for single 
            integration into the LEU2 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVl
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     252..269
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     253..269
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(298..314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(298..314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    315..348
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact
       61 ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat
      121 tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaagcg
      181 ttcatgacta aatgcttgca tcacaatact tgaacttgac aatattattt aaggacctat
      241 tgttttttcc atgtaaaacg acggccagtg catgcgtatc ggacgtctcc cttcatggtc
      301 atagctgttt cctgataact tcgtataatg tatgctatac gaagttattc tgtaggcgaa
      361 cctaaccggc caattttcca agaccgctct gccgctgagc ttgcacccgt gccgctacac
      421 aacacccaca catccgtaaa ccccccgcca tcggtatcac caaggttgtc tatgacgagg
      481 cgtacaagag gacgtggggg ccgcctctcc aatgtcacca ataggaaaaa agaaagaccg
      541 agtgcgagtt tctgacagaa gcctcaagaa ttttttttct ttcttcgact atgctggagg
      601 tagagatgat cgaggcagta gtcaagtata tataaattaa ttagttccac caccttcttt
      661 tcttgtgttg ctcctttcga tgctatttct ggctttttct aatttttttc atcgtttctt
      721 actttcttcc taatatataa attcttttgc attttcattc ttcctctcca tttgttcatt
      781 ctccttaaat tttttttagg tctcctttct ttcaagaaac acagtcatcg aactataatt
      841 aactaaacat gtcagcacct aagaagatag tagtattacc tggagaccac gtaggacagg
      901 agataacagc agaggcaata aaggtattaa aggcaatatc agacgtaagg tcaaacgtaa
      961 agttcgactt cgagaaccac ttaataggag gagcagcaat agacgcaaca ggagtacctt
     1021 t
//
