LOCUS       sequence-164491-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single Integration Vector LEU - Cloning vector for single 
            integration into the LEU2 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVl
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(107..170)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     448..465
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     449..465
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(494..510)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(494..510)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    511..544
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2390..2423
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2424..2443
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2424..2442
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2451..2558
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     2475..2491
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2475..2491
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2525..2541)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2526..2542)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(2571..2589)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(2878..3466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2958..2977)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3637..4391,1..37,38..106))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4260..4279
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
       61 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
      121 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaagtat
      181 cgttcgaatt aattaatttc ctcaacataa cgagaacaca caggggcgct atcgcacaga
      241 atcaaattcg atgactggaa attttttgtt aatttcagag gtcgcctgac gcatatacct
      301 ttttcaactg aaaaattggg agaaaaagga aaggtgagag cgccggaacc ggcttttcat
      361 atagaataga gaagcgttca tgactaaatg cttgcatcac aatacttgaa cttgacaata
      421 ttatttaagg acctattgtt ttttccatgt aaaacgacgg ccagtgcatg cgtatcggac
      481 gtctcccttc atggtcatag ctgtttcctg ataacttcgt ataatgtatg ctatacgaag
      541 ttattctgta ggcgaaccta accggccaat tttccaagac cgctctgccg ctgagcttgc
      601 acccgtgccg ctacacaaca cccacacatc cgtaaacccc ccgccatcgg tatcaccaag
      661 gttgtctatg acgaggcgta caagaggacg tgggggccgc ctctccaatg tcaccaatag
      721 gaaaaaagaa agaccgagtg cgagtttctg acagaagcct caagaatttt ttttctttct
      781 tcgactatgc tggaggtaga gatgatcgag gcagtagtca agtatatata aattaattag
      841 ttccaccacc ttcttttctt gtgttgctcc tttcgatgct atttctggct ttttctaatt
      901 tttttcatcg tttcttactt tcttcctaat atataaattc ttttgcattt tcattcttcc
      961 tctccatttg ttcattctcc ttaaattttt tttaggtctc ctttctttca agaaacacag
     1021 tcatcgaact ataattaact aaacatgtca gcacctaaga agatagtagt attacctgga
     1081 gaccacgtag gacaggagat aacagcagag gcaataaagg tattaaaggc aatatcagac
     1141 gtaaggtcaa acgtaaagtt cgacttcgag aaccacttaa taggaggagc agcaatagac
     1201 gcaacaggag tacctttacc tgacgaggca ttagaggcat caaagaaggc agacgcagta
     1261 ttattaggag cagtaggagg acctaagtgg ggaacaggat cagtaaggcc tgagcaggga
     1321 ttattaaaga taaggaagga gttacagtta tacgcaaact taaggccttg caacttcgca
     1381 tcagactcat tattagactt atcacctata aagcctcagt tcgcaaaggg aacagacttc
     1441 gtagtagtaa gggagttagt aggaggaata tacttcggaa agaggaagga ggacgacgga
     1501 gacggagtag catgggactc agagcagtac acagtacctg aggtacagag gataacaagg
     1561 atggcagcat tcatggcatt acagcacgag cctcctttac ctatatggtc attagacaag
     1621 gcaaacgtat tagcatcatc aaggttatgg aggaagacag tagaggagac aataaagaac
     1681 gagttcccta cattaaaggt acagcaccag ttaatagact cagcagcaat gatattagta
     1741 aagaacccta cacacttaaa cggaataata ataacatcaa acatgttcgg agacataata
     1801 tcagacgagg catcagtaat acctggatca ttaggattat taccttcagc atcattagca
     1861 tcattacctg acaagaacac agcattcgga ttatacgagc cttgccacgg atcagcacct
     1921 gacttaccta agaacaaggt aaaccctata gcaacaatat tatcagcagc aatgatgtta
     1981 aagttatcat taaacttacc tgaggaggga aaggcaatag aggacgcagt aaagaaggta
     2041 ttagacgcag gaataaggac aggagactta ggaggatcaa actcaacaac agaggtagga
     2101 gacgcagtag cagaggaggt aaagaagata ttagcatgag aatattgata aatttaaatt
     2161 ttggaatgag ggttgtcctt tcctttacaa tagaggcatt tgagtgagta ggttttatca
     2221 ccttcttgct cttattaaat cgcagttgtc tttccggtat gtgcttgact gaggaacaat
     2281 attttattca tgatacattt ccatgctacg catatcgtca tccacatgga aggtaatttt
     2341 cttctgtggc ctttcatata gttgtgaatg gtagaacaat cttcatataa taacttcgta
     2401 taatgtatgc tatacgaagt tattaatacg actcactata gggcgaattg gagctccacc
     2461 gcggtggcgg ccgctctaga actagtggat cccccgggct gcaggaattc gatatcaagc
     2521 ttatcgatac cgtcgacctc gagggggggc ccggtaccca gcttttgttc cctttagtga
     2581 gggttaattt cctgtaaact ttataaatga aattcataat agaaacgaca cgaaattaca
     2641 aaatggaata tgttcatagg gtagacgaaa ctatatacgc aatctacata catttatcaa
     2701 gaaggagaaa aaggaggatg taaaggaata caggtaagca aattgatact aatggctcaa
     2761 cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga gggcaccaca
     2821 caaaaagtta ggtgtaacag aaaatttaat taattcgaag gccgcgttgc tggcgttttt
     2881 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2941 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3001 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3061 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3121 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3181 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3241 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3301 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3361 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3421 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3481 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3541 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3601 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3661 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactgc ccgtcgtgta
     3721 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3781 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3841 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3901 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3961 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4021 gcgagttaca tgatccccca tgttgtgaaa aaaagcggtt agctccttcg gtcctccgat
     4081 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4141 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4201 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4261 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4321 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4381 acccaactga t
//
