LOCUS       sequence-164492-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single Integration Vector HIS - Cloning vector for single 
            integration into the HIS3 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVh
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     250..267
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     251..267
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(296..312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(296..312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    313..346
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agcgctagga gtcactgcca ggtatcgttt gaacacggca ttagtcaggg aagtcataac
       61 acagtccttt cccgcaattt tctttttcta ttactcttgg cctcctctag tacactctat
      121 atttttttat gcctcggtaa tgattttcat tttttttttt ccacctagcg gatgactctt
      181 tttttttctt agcgattggc attatcacat aatgaattat acattatata aagtaatgtg
      241 atttcttcgt gtaaaacgac ggccagtgca tgcgtatcgg acgtctccct tcatggtcat
      301 agctgtttcc tgataacttc gtataatgta tgctatacga agttatgtct gtaggcgaac
      361 ctaaccggcc aattttccaa gaccgctctg ccgctgagct tgcacccgtg ccgctacaca
      421 acacccacac atccgtaaac cccccgccat cggtatcacc aaggttgtct atgacgaggc
      481 gtacaagagg acgtgggggc cgcctctcca atgtcaccaa taggaaaaaa gaaagaccga
      541 gtgcgagttt ctgacagaag cctcaagaat tttttttctt tcttcgacta tgctggaggt
      601 agagatgatc gaggcagtag tcaagtatat ataaattaat tagttccacc accttctttt
      661 cttgtgttgc tcctttcgat gctatttctg gctttttcta atttttttca tcgtttctta
      721 ctttcttcct aatatataaa ttcttttgca ttttcattct tcctctccat ttgttcattc
      781 tccttaaatt ttttttaggt ctcctttctt tcaagaaaca cagtcatcga actataatta
      841 actaaacatg ggtaggaggg cttttgtaga aagaaatacg aacgaaacga aaatcagcgt
      901 tgctatcgct ttggacaaag ctcccttacc tgaagagtcg aattt
//
