LOCUS       sequence-164493-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single Integration Vector HIS - Cloning vector for single 
            integration into the HIS3 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVh
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     339..359
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        341..359
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    372..479
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     388..404
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     389..405
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(439..455)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(439..455)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(487..506)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(488..506)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(507..540)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(564..761)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttcga attaattaag gaataccact tgccacctat caccacaact
      121 aactttttcc cgttcctcca tctcttttat attttttttc tccagttcaa gagaaaaaaa
      181 aagaaaaagc aaaaagaaaa aaggaaagcg cgcctcgttc agaatgacac gtatagaatg
      241 atgcattacc ttgtcatctt cagtatcata ctgttcgtat acatacttac tgacattcat
      301 aggtatacat atatacacat gtatatatat cgtatgctgc aattaaccct cactaaaggg
      361 aacaaaagct gggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg
      421 aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
      481 aattcgccct atagtgagtc gtattaataa cttcgtatag catacattat acgaagttat
      541 cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg attgacgcat
      601 gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa
      661 atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca
      721 aatgacaagt tcttgaaaac aagaatcttt ttattgtcag taatgattac aacactccct
      781 tcgtgcttgg gacttcagaa cttccagtaa gacttgtagc cgcgcgcatg gcaacagcca
      841 gagatttaaa agcgctttca gcacgatgat ggtcattact accatataag caggtaacat
      901 gcaaagtaat tccagcagct accgaaaagg aatatagtaa gtgagggatc atttcacagg
      961 acaattcccc a
//
