LOCUS       sequence-164499-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector URA/YFP - Plasmid for monomeric Citrine 
            (mCitrine) labeling of endogenous proteins with a URA selection 
            marker, originating from pSIVu (ID:81089).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTU-y
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             51..761
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLKEF
                     VTAAGITHGMDELYK"
     terminator      806..993
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(861..885)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aattaaccct cactaaaggg gcatgcgcgg ccgctctaga actatctgct tctaaaggtg
       61 aagaattatt cactggtgtt gtcccaatct tggttgaatt agatggtgat gttaatggtc
      121 acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa
      181 aatttatttg tactactggt aaattgccag ttccatggcc aaccttagtc actactttag
      241 gttatggttt gatgtgtttt gctagatacc cagatcatat gaaacaacat gactttttca
      301 agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa gatgacggta
      361 actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat agaatcgaat
      421 taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg gaatacaact
      481 ataactctca caatgtttac atcatggctg acaaacaaaa gaatggtatc aaagttaact
      541 tcaaaattag acacaacatt gaagatggtt ctgttcaatt agctgaccat tatcaacaaa
      601 atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta tcctatcaat
      661 ctaaattatc caaagatcca aacgaaaaga gagaccacat ggtcttgaaa gaatttgtta
      721 ctgctgctgg tattacccat ggtatggatg aattgtacaa aggaatgcta gcattttaac
      781 tcgagggcgc gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
      841 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
      901 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta
      961 tgaggtcgct cttattgacc acacctctac cggcagatcc gctaggga
//
