LOCUS       sequence-164501-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector URA/RFP - Plasmid for monomeric Cherry (mCherry)
            labeling of endogenous proteins with a URA selection marker, 
            originating from pSIVu (ID:81089).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTU-r
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(181..199)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     449..468
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      794..981
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(849..873)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aattaaccct cactaaaggg gcatgcgcgg ccgctctaga actaaagggc gaggaggata
       61 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      121 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      181 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      241 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      301 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      361 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      421 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      481 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
      541 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
      601 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
      661 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
      721 gcggcatgga cgagctgtac aagggatctt aaatgctagc attttaactc gagggcgcgc
      781 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
      841 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
      901 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct
      961 tattgaccac acctctaccg gca
//
