LOCUS       sequence-164506-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector LEU/CFP - Plasmid for yeast enhanced monomeric 
            CFP (yemCFP) labeling of endogenous proteins with a LEU selection 
            marker, originating from pSIVl (ID:81090).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTL-c
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             51..767
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     terminator      811..998
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(866..890)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aattaaccct cactaaaggg gcatgcgcgg ccgctctaga actaattaac atgtctaaag
       61 gtgaagaatt attcactggt gttgtcccaa ttttggttga attagatggt gatgttaatg
      121 gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt aaattgacct
      181 taaaatttat ttgtactact ggtaaattgc cagttccatg gccaacctta gtcactactt
      241 taacttgggg tgttcaatgt ttttctagat acccagatca tatgaaacaa catgactttt
      301 tcaagtctgc catgccagaa ggttatgttc aagaaagaac tatttttttc aaagatgacg
      361 gtaactacaa gaccagagct gaagtcaagt ttgaaggtga taccttagtt aatagaatcg
      421 aattaaaagg tattgatttt aaagaagatg gtaacatttt aggtcacaaa ttggaataca
      481 tttataactc tcacaatgtt tacatcactg ctgacaaaca aaagaatggt atcaaagcta
      541 acttcaaaat tagacacaac attgaagatg gttctgttca attagctgac cattatcaac
      601 aaaatactcc aattggtgat ggtccagtct tgttaccaga caaccattac ttatccactc
      661 aatctagatt atccaaagat ccaaacgaaa agagagacca catggtcttg ttagaatttg
      721 ttactgctgc tggtattacc catggtattg atgaattgta caaataagga tgctagcatt
      781 ttaactcgag ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt
      841 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
      901 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
      961 tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag gg
//
