LOCUS       sequence-164507-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector LEU/CFP - Plasmid for yeast enhanced monomeric 
            CFP (yemCFP) labeling of endogenous proteins with a LEU selection 
            marker, originating from pSIVl (ID:81090).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTL-c
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(132..150)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(151..184)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc ttcgaattaa ttaataatac gactcactat aggggacgtc cgaattggag
      121 ctccaattcg ccctatagtg agtcgtatta ataacttcgt atagcataca ttatacgaag
      181 ttattatatg aagattgttc taccattcac aactatatga aaggccacag aagaaaatta
      241 ccttccatgt ggatgacgat atgcgtagca tggaaatgta tcatgaataa aatattgttc
      301 ctcagtcaag cacataccgg aaagacaact gcgatttaat aagagcaaga aggtgataaa
      361 acctactcac tcaaatgcct ctattgtaaa ggaaaggaca accctcattc caaaatttaa
      421 atttatcaat attctcatgc taatatcttc tttacctcct ctgctactgc gtctcctacc
      481 tctgttgttg agtttgatcc tcctaagtct cctgtcctta ttcctgcgtc taataccttc
      541 tttactgcgt cctctattgc ctttccctcc tcaggtaagt ttaatgataa ctttaacatc
      601 attgctgctg ataatattgt tgctataggg tttaccttgt tcttaggtaa gtcaggtgct
      661 gatccgtggc aaggctcgta taatccgaat gctgtgttct tgtcaggtaa tgatgctaat
      721 gatgctgaag gtaataatcc taatgatcca ggtattactg atgcctcgtc tgatattatg
      781 tctccgaaca tgtttgatgt tattattatt ccgtttaagt gtgtagggtt ctttactaat
      841 atcattgctg ctgagtctat taactggtgc tgtaccttta atgtagggaa ctcgttcttt
      901 attgtctcct ctactgtctt cctccataac cttgatgatg ctaatacgtt t
//
