LOCUS       sequence-164508-        4774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector LEU/CFP - Plasmid for yeast enhanced monomeric 
            CFP (yemCFP) labeling of endogenous proteins with a LEU selection 
            marker, originating from pSIVl (ID:81090).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTL-c
FEATURES             Location/Qualifiers
     source          1..4774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(28..91)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        118..136
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             168..884
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     terminator      928..1115
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(983..1007)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1154..1170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1154..1170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1171..1204
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3050..3083
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3084..3102
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3131..3149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3182..3770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3262..3281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3941..4732,4733..4774,1..27))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4564..4583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
       61 tcagggttat tgtctcatga gcggatacat atttgaagta tcgttcgaat taattaaaat
      121 taaccctcac taaaggggca tgcgcggccg ctctagaact aattaacatg tctaaaggtg
      181 aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat gttaatggtc
      241 acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa
      301 aatttatttg tactactggt aaattgccag ttccatggcc aaccttagtc actactttaa
      361 cttggggtgt tcaatgtttt tctagatacc cagatcatat gaaacaacat gactttttca
      421 agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa gatgacggta
      481 actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat agaatcgaat
      541 taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg gaatacattt
      601 ataactctca caatgtttac atcactgctg acaaacaaaa gaatggtatc aaagctaact
      661 tcaaaattag acacaacatt gaagatggtt ctgttcaatt agctgaccat tatcaacaaa
      721 atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta tccactcaat
      781 ctagattatc caaagatcca aacgaaaaga gagaccacat ggtcttgtta gaatttgtta
      841 ctgctgctgg tattacccat ggtattgatg aattgtacaa ataaggatgc tagcatttta
      901 actcgagggc gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt
      961 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
     1021 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
     1081 tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga taacagggta
     1141 atatagatcc atggtcatag ctgtttcctg ataacttcgt ataatgtatg ctatacgaag
     1201 ttattctgta ggcgaaccta accggccaat tttccaagac cgctctgccg ctgagcttgc
     1261 acccgtgccg ctacacaaca cccacacatc cgtaaacccc ccgccatcgg tatcaccaag
     1321 gttgtctatg acgaggcgta caagaggacg tgggggccgc ctctccaatg tcaccaatag
     1381 gaaaaaagaa agaccgagtg cgagtttctg acagaagcct caagaatttt ttttctttct
     1441 tcgactatgc tggaggtaga gatgatcgag gcagtagtca agtatatata aattaattag
     1501 ttccaccacc ttcttttctt gtgttgctcc tttcgatgct atttctggct ttttctaatt
     1561 tttttcatcg tttcttactt tcttcctaat atataaattc ttttgcattt tcattcttcc
     1621 tctccatttg ttcattctcc ttaaattttt tttaggtctc ctttctttca agaaacacag
     1681 tcatcgaact ataattaact aaacatgtca gcacctaaga agatagtagt attacctgga
     1741 gaccacgtag gacaggagat aacagcagag gcaataaagg tattaaaggc aatatcagac
     1801 gtaaggtcaa acgtaaagtt cgacttcgag aaccacttaa taggaggagc agcaatagac
     1861 gcaacaggag tacctttacc tgacgaggca ttagaggcat caaagaaggc agacgcagta
     1921 ttattaggag cagtaggagg acctaagtgg ggaacaggat cagtaaggcc tgagcaggga
     1981 ttattaaaga taaggaagga gttacagtta tacgcaaact taaggccttg caacttcgca
     2041 tcagactcat tattagactt atcacctata aagcctcagt tcgcaaaggg aacagacttc
     2101 gtagtagtaa gggagttagt aggaggaata tacttcggaa agaggaagga ggacgacgga
     2161 gacggagtag catgggactc agagcagtac acagtacctg aggtacagag gataacaagg
     2221 atggcagcat tcatggcatt acagcacgag cctcctttac ctatatggtc attagacaag
     2281 gcaaacgtat tagcatcatc aaggttatgg aggaagacag tagaggagac aataaagaac
     2341 gagttcccta cattaaaggt acagcaccag ttaatagact cagcagcaat gatattagta
     2401 aagaacccta cacacttaaa cggaataata ataacatcaa acatgttcgg agacataata
     2461 tcagacgagg catcagtaat acctggatca ttaggattat taccttcagc atcattagca
     2521 tcattacctg acaagaacac agcattcgga ttatacgagc cttgccacgg atcagcacct
     2581 gacttaccta agaacaaggt aaaccctata gcaacaatat tatcagcagc aatgatgtta
     2641 aagttatcat taaacttacc tgaggaggga aaggcaatag aggacgcagt aaagaaggta
     2701 ttagacgcag gaataaggac aggagactta ggaggatcaa actcaacaac agaggtagga
     2761 gacgcagtag cagaggaggt aaagaagata ttagcatgag aatattgata aatttaaatt
     2821 ttggaatgag ggttgtcctt tcctttacaa tagaggcatt tgagtgagta ggttttatca
     2881 ccttcttgct cttattaaat cgcagttgtc tttccggtat gtgcttgact gaggaacaat
     2941 attttattca tgatacattt ccatgctacg catatcgtca tccacatgga aggtaatttt
     3001 cttctgtggc ctttcatata gttgtgaatg gtagaacaat cttcatataa taacttcgta
     3061 taatgtatgc tatacgaagt tattaatacg actcactata gggcgaattg gagctccaat
     3121 tcggacgtcc cctatagtga gtcgtattat taattaattc gaaggccgcg ttgctggcgt
     3181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctgcccgtcg
     4021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4321 caaggcgagt tacatgatcc cccatgttgt gaaaaaaagc ggttagctcc ttcggtcctc
     4381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4741 caggaaggca aaatgccgca aaaaagggaa taag
//
