LOCUS       sequence-164510-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) 
            labeling of endogenous proteins with a LEU selection marker, 
            originating from pSIVl (ID:81090).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTL-g
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        89..107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(136..154)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(155..188)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttcgaa ttaattaata atacgactca ctatagggga cgtccgaatt
      121 ggagctccaa ttcgccctat agtgagtcgt attaataact tcgtatagca tacattatac
      181 gaagttatta tatgaagatt gttctaccat tcacaactat atgaaaggcc acagaagaaa
      241 attaccttcc atgtggatga cgatatgcgt agcatggaaa tgtatcatga ataaaatatt
      301 gttcctcagt caagcacata ccggaaagac aactgcgatt taataagagc aagaaggtga
      361 taaaacctac tcactcaaat gcctctattg taaaggaaag gacaaccctc attccaaaat
      421 ttaaatttat caatattctc atgctaatat cttctttacc tcctctgcta ctgcgtctcc
      481 tacctctgtt gttgagtttg atcctcctaa gtctcctgtc cttattcctg cgtctaatac
      541 cttctttact gcgtcctcta ttgcctttcc ctcctcaggt aagtttaatg ataactttaa
      601 catcattgct gctgataata ttgttgctat agggtttacc ttgttcttag gtaagtcagg
      661 tgctgatccg tggcaaggct cgtataatcc gaatgctgtg ttcttgtcag gtaatgatgc
      721 taatgatgct gaaggtaata atcctaatga tccaggtatt actgatgcct cgtctgatat
      781 tatgtctccg aacatgtttg atgttattat tattccgttt aagtgtgtag ggttctttac
      841 taatatcatt gctgctgagt ctattaactg gtgctgtacc tttaatgtag ggaactcgtt
      901 ctt
//
