LOCUS       sequence-164512-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 
            cloning of inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: MCV
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    44..151
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     60..76
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     61..77
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(111..127)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(111..127)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(165..184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(166..184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(217..805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(297..316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaattaaccc tcactaaagg ggcatgcaac aaaagctggg tacggtaccg ggccccccct
       61 cgaggtcgac ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag
      121 ttctagagcg gccgccaccg cggtggagct ccaattcgga cgtcccctat agtgagtcgt
      181 attattaatt aattcgaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      241 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      301 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      361 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      421 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      481 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      541 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      601 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      661 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      721 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      781 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      841 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
      901 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
      961 aacttggtct gacagttacc aatgcttaat cagtga
//
