LOCUS       sequence-164514-        1925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 
            cloning of inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: MCV
FEATURES             Location/Qualifiers
     source          1..1925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(108..127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(787..1578,1579..1647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1410..1429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1648..1711)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1738..1756
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1780..1887
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     1796..1812
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1797..1813
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1847..1863)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1847..1863)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1901..1920)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1902..1920)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taattcgaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
       61 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
      841 atccatagtt gcctgactgc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1681 tcagggttat tgtctcatga gcggatacat atttgaagta tcgttcgaat taattaaaat
     1741 taaccctcac taaaggggca tgcaacaaaa gctgggtacg gtaccgggcc ccccctcgag
     1801 gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cactagttct
     1861 agagcggccg ccaccgcggt ggagctccaa ttcggacgtc ccctatagtg agtcgtatta
     1921 ttaat
//
