LOCUS       sequence-164523-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector LEU/RFP - Plasmid for monomeric Cherry (mCherry)
            labeling of endogenous proteins with a LEU selection marker, 
            originating from pSIVl (ID:81090).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTL-r
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        90..108
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(137..155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(156..189)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttcga attaattaat aatacgactc actatagggg acgtccgaat
      121 tggagctcca attcgcccta tagtgagtcg tattaataac ttcgtatagc atacattata
      181 cgaagttatt atatgaagat tgttctacca ttcacaacta tatgaaaggc cacagaagaa
      241 aattaccttc catgtggatg acgatatgcg tagcatggaa atgtatcatg aataaaatat
      301 tgttcctcag tcaagcacat accggaaaga caactgcgat ttaataagag caagaaggtg
      361 ataaaaccta ctcactcaaa tgcctctatt gtaaaggaaa ggacaaccct cattccaaaa
      421 tttaaattta tcaatattct catgctaata tcttctttac ctcctctgct actgcgtctc
      481 ctacctctgt tgttgagttt gatcctccta agtctcctgt ccttattcct gcgtctaata
      541 ccttctttac tgcgtcctct attgcctttc cctcctcagg taagtttaat gataacttta
      601 acatcattgc tgctgataat attgttgcta tagggtttac cttgttctta ggtaagtcag
      661 gtgctgatcc gtggcaaggc tcgtataatc cgaatgctgt gttcttgtca ggtaatgatg
      721 ctaatgatgc tgaaggtaat aatcctaatg atccaggtat tactgatgcc tcgtctgata
      781 ttatgtctcc gaacatgttt gatgttatta ttattccgtt taagtgtgta gggttcttta
      841 ctaatatcat tgctgctgag tctattaact ggtgctgtac ctttaatgta gggaactcgt
      901 tctttattgt ctcctctact g
//
