LOCUS       sequence-164525-        3564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing AKT1S1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_AKT1S1_WT
FEATURES             Location/Qualifiers
     source          1..3564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1443..1542)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1559..1578)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1560..1578)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1583..1599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1583..1599)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2030..2821
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2914..3502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3403..3422
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggcgtcgg ggcgccccga ggagctgtgg gaggccgtgg tgggggccgc
      721 tgagcgcttc cgggcccgga ctggcacgga gctggtgctg ctgaccgcgg ccccgccgcc
      781 accaccccgc ccgggcccct gtgcctatcc tgcccatggt cgaggagccc tggcggaggc
      841 agcgcgccgt tgcctccacg acatcgcact ggcccacagg gctgccactg ctgctcggcc
      901 tcctgcgccc ccaccagcac cacagccacc cagtcccaca cccagcccac cccggcctac
      961 cctggccaga gaggacaacg aggaggacga ggatgagccc acagagacag agacctccgg
     1021 ggagcagctg ggcattagtg ataatggagg gctctttgtg atggatgagg acgccaccct
     1081 ccaggacctt ccccccttct gtgagtcaga ccccgagagt acagatgatg gcagcctgag
     1141 cgaggagacc cccgccggcc cccccacctg ctcagtgccc ccagcctcag ccctacccac
     1201 acagcagtac gccaagtccc tgcctgtgtc tgtgcccgtc tggggcttca aggagaagag
     1261 gacagaggcg cggtcatcag atgaggagaa tgggccgccc tcttcgcccg acctggaccg
     1321 catcgcggcg agcatgcgcg cgctggtgct gcgagaggcc gaggacaccc aggtcttcgg
     1381 ggacctgcca cggccgcggc ttaacaccag cgacttccag aagctgaagc ggaaatattg
     1441 attgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1501 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     1561 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     1621 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     1681 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     1741 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     1801 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     1861 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     1921 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1981 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     2041 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2101 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2161 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2221 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2281 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2341 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2401 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2461 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2521 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2581 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2641 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2701 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2761 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     2821 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     2881 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2941 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3001 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3061 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3121 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3181 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3241 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3301 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3361 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3421 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3481 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3541 ttgctggcct tttgctcaca tgtt
//
