LOCUS       sequence-164526-         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CPH1S-o domain (Synechocystis Phytochrome 1) preceded by a
            MYR signal and a restriction site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Reichhart E, Ingles-Prieto A, Tichy AM, McKenzie C, Janovjak H
  TITLE     A Phytochrome Sensory Domain Permits Receptor Activation by Red 
            Light.
  JOURNAL   Angew Chem Int Ed Engl. 2016 May 17;55(21):6339-42. doi: 
            10.1002/anie.201601736. Epub 2016 Apr 21.
  PUBMED    27101018
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/anie.201601736"; journalName: "Angew Chem Int Ed Engl"; 
            date: "2016-05-17- 17"; volume: "55"; issue: "21"; pages: "6339-42"
COMMENT     Sequence Label: MYR-CPH1S-o_445
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             122..163
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
       61 gggagaccca agctggctag cgtttaaacg ggccctctag actcgagcgg ccgcggccac
      121 catggggagt agcaagagca agcctaagga ccccagccag cgcctcgacg tcaccggtgg
      181 agcaactact gttcaactgt ctgatcaatc tctgcgtcaa ctggaaactc tggctatcca
      241 caccgcgcat ctgatccagc cgcacggtct ggtagtcgtc ctgcaagaac cggacctgac
      301 catcagccag atctctgcga actgtaccgg tatcctgggc cgtagcccgg aagatctgct
      361 gggtcgtact ctgggcgagg tattcgattc ttttcagatt gatccgatcc agtctcgtct
      421 gaccgcaggt cagatttcca gcctgaaccc gtccaagctg tgggcgcgtg ttatgggtga
      481 cgactttgtt attttcgacg gcgtatttca tcgtaactct gatggcctgc tggtttgcga
      541 gctggagccg gcctacacta gcgacaacct gcctttcctg ggtttctacc atatggcaaa
      601 cgcggcactg aaccgtctgc g
//
