LOCUS       sequence-164534-        4348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector containing serum response element (SRE) followed by 
            an AscI restriction site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Reichhart E, Ingles-Prieto A, Tichy AM, McKenzie C, Janovjak H
  TITLE     A Phytochrome Sensory Domain Permits Receptor Activation by Red 
            Light.
  JOURNAL   Angew Chem Int Ed Engl. 2016 May 17;55(21):6339-42. doi: 
            10.1002/anie.201601736. Epub 2016 Apr 21.
  PUBMED    27101018
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/anie.201601736"; journalName: "Angew Chem Int Ed Engl"; 
            date: "2016-05-17- 17"; volume: "55"; issue: "21"; pages: "6339-42"
COMMENT     Sequence Label: SRE reporter vector_559
FEATURES             Location/Qualifiers
     source          1..4348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        179..210
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    303..424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(314..333)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     368..387
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        618..975
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        618..809
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(641..661)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      826..961
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     888..907
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1006..2043
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             2022..2030
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    2067..2115
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(2271..2288)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2442..3030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2522..2541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3230..4021,4022..4090))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4195..4243
                     /note="synthetic polyadenylation signal"
     misc_feature    4257..4348
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4297..4316
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcaggatgtc catattagga catctaggat
       61 gtccatatta ggacatctag gatgtccata ttaggacatc taggatgtcc atattaggac
      121 atctaggatg tccatattag gacatctaga tctggcctcg gcggccaagc ttagacacta
      181 gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca
      241 ccggcgcgcc ctaaggccgc gactctagag tcggggcggc cggccgcttc gagcagacat
      301 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
      361 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
      421 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
      481 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtttgcgta
      541 ttgggcgctc ttccgctgat ctgcgcagca ccatggcctg aaataacctc tgaaagagga
      601 acttggttag ctaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
      661 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      721 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      781 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
      841 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      901 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      961 ctaggctttt gcaaaaagct cgattcttct gacactagcg ccaccatgaa gaagcccgaa
     1021 ctcaccgcta ccagcgttga aaaatttctc atcgagaagt tcgacagtgt gagcgacctg
     1081 atgcagttgt cggagggcga agagagccga gccttcagct tcgatgtcgg cggacgcggc
     1141 tatgtactgc gggtgaatag ctgcgctgat ggcttctaca aagaccgcta cgtgtaccgc
     1201 cacttcgcca gcgctgcact acccatcccc gaagtgttgg acatcggcga gttcagcgag
     1261 agcctgacat actgcatcag tagacgcgcc caaggcgtta ctctccaaga cctccccgaa
     1321 acagagctgc ctgctgtgtt acagcctgtc gccgaagcta tggatgctat tgccgccgcc
     1381 gacctcagtc aaaccagcgg cttcggccca ttcgggcccc aaggcatcgg ccagtacaca
     1441 acctggcggg atttcatttg cgccattgct gatccccatg tctaccactg gcagaccgtg
     1501 atggacgaca ccgtgtccgc cagcgtagct caagccctgg acgaactgat gctgtgggcc
     1561 gaagactgtc ccgaggtgcg ccacctcgtc catgccgact tcggcagcaa caacgtcctg
     1621 accgacaacg gccgcatcac cgccgtaatc gactggtccg aagctatgtt cggggacagt
     1681 cagtacgagg tggccaacat cttcttctgg cggccctggc tggcttgcat ggagcagcag
     1741 actcgctact tcgagcgccg gcatcccgag ctggccggca gccctcgtct gcgagcctac
     1801 atgctgcgca tcggcctgga tcagctctac cagagcctcg tggacggcaa cttcgacgat
     1861 gctgcctggg ctcaaggccg ctgcgatgcc atcgtccgca gcggggccgg caccgtcggt
     1921 cgcacacaaa tcgctcgccg gagcgcagcc gtatggaccg acggctgcgt cgaggtgctg
     1981 gccgacagcg gcaaccgccg gcccagtaca cgaccgcgcg ctaaggaggt aggtcgagtt
     2041 taaactctag aaccggtcat ggccgcaata aaatatcttt attttcatta catctgtgtg
     2101 ttggtttttt gtgtgttcga actagatgct gtcgaccgat gcccttgaga gccttcaacc
     2161 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     2221 tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact
     2281 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2341 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2401 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2461 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2521 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2581 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2641 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2701 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2761 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2821 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2881 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2941 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3001 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3061 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3121 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3181 gagtaaactt ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt taccagtgct
     3241 tgatcagtga ggcaccgatc tcagcgatct gcctatttcg ttcgtccata gtggcctgac
     3301 tccccgtcgt gtagatcact acgattcgtg agggcttacc atcaggcccc agcgcagcaa
     3361 tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc agcaatgaac cagccagcag
     3421 ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag tctatgagct
     3481 gctgtcgtga tgctagagta agaagttcgc cagtgagtag tttccgaaga gttgtggcca
     3541 ttgctactgg catcgtggta tcacgctcgt cgttcggtat ggcttcgttc aactctggtt
     3601 cccagcggtc aagccgggtc acatgatcac ccatattatg aagaaatgca gtcagctcct
     3661 tagggcctcc gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc atggtaatgg
     3721 cagcactaca caattctctt accgtcatgc catccgtaag atgcttttcc gtgaccggcg
     3781 agtactcaac caagtcgttt tgtgagtagt gtatacggcg accaagctgc tcttgcccgg
     3841 cgtctatacg ggacaacacc gcgccacata gcagtacttt gaaagtgctc atcatcggga
     3901 atcgttcttc ggggcggaaa gactcaagga tcttgccgct attgagatcc agttcgatat
     3961 agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc gtttcggggt
     4021 gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt
     4081 ggatgctcat actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg
     4141 tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa
     4201 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca
     4261 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt
     4321 gcaagtgcag gtgccagaac atttctct
//
