LOCUS       sequence-164535-         555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil peptide SYNZIP10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pENTR-SYNZIP10
FEATURES             Location/Qualifiers
     source          1..555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..83
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(261..360)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(377..396)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(378..396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(401..417)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(401..417)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(519..538)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
       61 aactttgtac aaaaaagcag gctccgcggc cgcccccttc accatgggat ccaacctgct
      121 ggcgaccctg cgttctaccg ctgcggttct ggaaaacgaa aaccacgtac tggagaagga
      181 gaaagagaaa ctgcgcaaag aaaaagaaca gctgctgaac aaactggaag cgtacaaatg
      241 ataaaagggt gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca
      301 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
      361 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
      421 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      481 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
      541 tcaacgggaa acgtc
//
