LOCUS       sequence-164536-        5304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing AR , part of the Target Accelerator 
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_AR_WT
FEATURES             Location/Qualifiers
     source          1..5304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3183..3282)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3299..3318)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3300..3318)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3323..3339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3323..3339)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3770..4561
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4654..5242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5143..5162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggaagtgc agttagggct gggaagggtc taccctcggc cgccgtctaa
      721 gacctaccga ggagctttcc agaatctgtt ccagagcgtg cgcgaagtga tccagaaccc
      781 gggccccagg cacccagagg ccgcgagcgc agcacctccc ggcgccagtt tgctgctgct
      841 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
      901 gcagcagcag cagcaagaga ctagccccag gcagcagcag cagcagcagg gtgaggatgg
      961 ttctccccaa gcccatcgta gaggccccac aggctacctg gtcctggatg aggaacagca
     1021 accttcacag ccgcagtcgg ccctggagtg ccaccccgag agaggttgcg tcccagagcc
     1081 tggagccgcc gtggccgcca gcaaggggct gccgcagcag ctgccagcac ctccggacga
     1141 ggatgactca gctgccccat ccacgttgtc cctgctgggc cccactttcc ccggcttaag
     1201 cagctgctcc gctgacctta aagacatcct gagcgaggcc agcaccatgc aactccttca
     1261 gcaacagcag caggaagcag tatccgaagg cagcagcagc gggagagcga gggaggcctc
     1321 gggggctccc acttcctcca aggacaatta cttagggggc acttcgacca tttctgacaa
     1381 cgccaaggag ttgtgtaagg cagtgtcggt gtccatgggc ctgggtgtgg aggcgttgga
     1441 gcatctgagt ccaggggaac agcttcgggg ggattgcatg tacgccccac ttttgggagt
     1501 tccacccgct gtgcgtccca ctccttgtgc cccattggcc gaatgcaaag gttctctgct
     1561 agacgacagc gcaggcaaga gcactgaaga tactgctgag tattcccctt tcaagggagg
     1621 ttacaccaaa gggctagaag gcgagagcct aggctgctct ggcagcgctg cagcagggag
     1681 ctccgggaca cttgaactgc cgtctaccct gtctctctac aagtccggag cactggacga
     1741 ggcagctgcg taccagagtc gcgactacta caactttcca ctggctctgg ccggaccgcc
     1801 gccccctccg ccgcctcccc atccccacgc tcgcatcaag ctggagaacc cgctggacta
     1861 cggctacact cggccccctc aggggctggc gggccaggaa agcgacttca ccgcacctga
     1921 tgtgtggtac cctggcggca tggtgagcag agtgccctat cccagtccca cttgtgtcaa
     1981 aagcgaaatg ggcccctgga tggatagcta ctccggacct tacggggaca tgcgtttgga
     2041 gactgccagg gaccatgttt tgcccattga ctattacttt ccaccccaga agacctgcct
     2101 gatctgtgga gatgaagctt ctgggtgtca ctatggagct ctcacatgtg gaagctgcaa
     2161 ggtcttcttc aaaagagccg ctgaagggaa acagaagtac ctgtgcgcca gcagaaatga
     2221 ttgcactatt gataaattcc gaaggaaaaa ttgtccatct tgtcgtcttc ggaaatgtta
     2281 tgaagcaggg atgactctgg gagcccggaa gctgaagaaa cttggtaatc tgaaactaca
     2341 ggaggaagga gaggcttcca gcaccaccag ccccactgag gagacaaccc agaagctgac
     2401 agtgtcacac attgaaggct atgaatgtca gcccatcttt ctgaatgtcc tggaagccat
     2461 tgagccaggt gtagtgtgtg ctggacacga caacaaccag cccgactcct ttgcagcctt
     2521 gctctctagc ctcaatgaac tgggagagag acagcttgta cacgtggtca agtgggccaa
     2581 ggccttgcct ggcttccgca acttacacgt ggacgaccag atggctgtca ttcagtactc
     2641 ctggatgggg ctcatggtgt ttgccatggg ctggcgatcc ttcaccaatg tcaactccag
     2701 gatgctctac ttcgcccctg atctggtttt caatgagtac cgcatgcaca agtcccggat
     2761 gtacagccag tgtgtccgaa tgaggcacct ctctcaagag tttggatggc tccaaatcac
     2821 cccccaggaa ttcctgtgca tgaaagcact gctactcttc agcattattc cagtggatgg
     2881 gctgaaaaat caaaaattct ttgatgaact tcgaatgaac tacatcaagg aactcgatcg
     2941 tatcattgca tgcaaaagaa aaaatcccac atcctgctca agacgcttct accagctcac
     3001 caagctcctg gactccgtgc agcctattgc gagagagctg catcagttca cttttgacct
     3061 gctaatcaag tcacacatgg tgagcgtgga ctttccggaa atgatggcag agatcatctc
     3121 tgtgcaagtg cccaagatcc tttctgggaa agtcaagccc atctatttcc acacccagtg
     3181 attgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     3241 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     3301 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     3361 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     3421 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     3481 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     3541 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     3601 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     3661 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     3721 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     3781 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     3841 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     3901 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     3961 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     4021 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     4081 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     4141 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     4201 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     4261 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     4321 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     4381 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     4441 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     4501 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     4561 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     4621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4801 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5041 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5221 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5281 ttgctggcct tttgctcaca tgtt
//
