LOCUS       sequence-164538-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Doench JG, Sharp PA
  TITLE     Specificity of microRNA target selection in translational 
            repression.
  JOURNAL   Genes Dev. 2004 Mar 1. 18(5):504-11.
  PUBMED    15014042
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2004 Mar 1. 18(5):504-11."
COMMENT     Sequence Label: pRL-TK let7 H
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          109..241
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     224..243
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        286..304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtggctcgac ccgaagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca
       61 acagtctcga ncttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      121 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      181 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      241 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
      301 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac
      361 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta
      421 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc
      481 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc
      541 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga
      601 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt
      661 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa
      721 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg
      781 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaaggagaa aaaatg
//
