LOCUS       sequence-164542-         524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil peptide SYNZIP13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pENTR-SYNZIP13
FEATURES             Location/Qualifiers
     source          1..524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(280..379)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(395..412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(395..411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(404..426)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      475..521
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtcggc gcgcccaccc ttttatcatt
      121 caaattcgtg agccgccagt tcgtctttca gataagcgat ttcctgtttc aggtgcgcaa
      181 cacggttctt tttaaccgcg ttacggtttt ccagttccgc gattttgttt ttcagttctt
      241 caactttctg ggatcccatg gtgaaggggg cggccgcgga gcctgctttt ttgtacaaag
      301 ttggcattat aaaaaagcat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc
      361 aaaataaaat cattatttgg ggcccgagct taagactggc cgtcgtttta caacgtcgtg
      421 actgggaaaa catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata
      481 aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tctg
//
